![]() To include the annotation, you need the sequence in Genbank format:Ĭopy the text and paste it in Notepad. Open the annotated sequence of mRFP-Rab5 in SnapGene.Ĭlick the Open button in the top menu, browse to the mRFPrab5.txt file and open it. Since it's a vector sequence, select circular:Ĭlick OK to open the sequence in SnapGene.Ĭhanging annotation of sequences in SnapGeneĪlthough we imported mRFP1-Rab5 in Genbank format, the annotation that was included in the file was not complete: SnapGene will ask you wether the sequence is linear or circular. what is called ORF frame 2 in the map is a neomycin/kanamycin resistance gene. ![]() ![]() What is called ORF frame 1 in the map is a fusion between mRFP1 (a red fluorescent protein) and human Rab5 (a small GTPase that is a key regulator of intracellular membrane trafficking) gene.
0 Comments
Leave a Reply. |